Regulation of Bacillus subtilis aprE Expression by glnA through Inhibition of scoC and -Dependent degR Expression
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Interplay of CodY and ScoC in the Regulation of Major Extracellular Protease Genes of Bacillus subtilis.
UNLABELLED AprE and NprE are two major extracellular proteases in Bacillus subtilis whose expression is directly regulated by several pleiotropic transcriptional factors, including AbrB, DegU, ScoC, and SinR. In cells growing in a rich, complex medium, the aprE and nprE genes are strongly expressed only during the post-exponential growth phase; mutations in genes encoding the known regulators a...
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in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
متن کاملCorrelation between Bacillus subtilis scoC phenotype and gene expression determined using microarrays for transcriptome analysis.
The availability of the complete sequence of the Bacillus subtilis chromosome (F. Kunst et al., Nature 390:249-256, 1997) makes possible the construction of genome-wide DNA arrays and the study of this organism on a global scale. Because we have a long-standing interest in the effects of scoC on late-stage developmental phenomena as they relate to aprE expression, we studied the genome-wide eff...
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Bacillus is remarkable for its extracellular enzyme production (Priest, 1977). When Bacillus cells exhaust the nutrients of the environment and enter into the stationary phase, neutral and alkaline proteases encoded by nprE and aprE respectively are produced and secreted to the outside environment (Chaloupka and Kreckova, 1966; Din et al., 1969). The mechanism of aprE expression has been extens...
متن کاملHpr (ScoC) and the phosphorelay couple cell cycle and sporulation in Bacillus subtilis.
Bacillus subtilis sporulation is a developmental process that culminates in the formation of a highly resistant and persistent endospore. Inhibiting DNA synthesis prior to the completion of the final round of DNA replication blocks sporulation at an early stage. Conditions that prevent compartmentalization of gene expression, i.e. inhibition of asymmetric septum formation or chromosome partitio...
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تاریخ انتشار 2009